[1]杨 帆,孟章进,姜桂英,等.除硫菌剂的工业化生产条件研究与应用[J].复杂油气藏,2021,14(04):116-120.[doi:10.16181/j.cnki.fzyqc.2021.04.020]
 YANG Fan,MENG ZhangJin,JIANG Guiying,et al.Study and application of industrialized production conditions of sulfurremoval bacteria[J].Complex Hydrocarbon Reservoirs,2021,14(04):116-120.[doi:10.16181/j.cnki.fzyqc.2021.04.020]
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除硫菌剂的工业化生产条件研究与应用()
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《复杂油气藏》[ISSN:1674-4667/CN:31-2019/TQ]

卷:
14卷
期数:
2021年04期
页码:
116-120
栏目:
油气工程
出版日期:
2021-12-25

文章信息/Info

Title:
Study and application of industrialized production conditions of sulfurremoval bacteria
作者:
杨 帆12孟章进12姜桂英1钱志鸿12王志明1
1.中国石化江苏油田分公司工程技术研究院,江苏 扬州,225009;2:江苏省油气微生物工程研究中心,江苏 扬州,225009
Author(s):
YANG Fan12MENG ZhangJin12JIANG Guiying1QIAN Zhihong12WANG Zhiming1
1. Petroleum Engineering Technology Research Institute of Jiangsu Oilfield Company,Yangzhou 225009,China;2. Jiangsu Oil and Gas Microbial Engineering Research Center,Yangzhou 225009,China
关键词:
除硫菌培养基工业发酵
Keywords:
sulfur removal bacteriaculture mediumindustrial fermentation
分类号:
Q939.97
DOI:
10.16181/j.cnki.fzyqc.2021.04.020
文献标志码:
A
摘要:
除硫菌N411是一种可以高效除硫的高温反硝化细菌,为提高该菌的培养效能,提高发酵水平,开展了工业发酵培养基优化研究和工业生产条件研究。通过调整培养基中碳和氮的比例,对基础培养基进行优化,从4组配方中优化出最优培养基,考察不同温度、pH值和装液量对除硫菌生长状况的影响,从而确定工业发酵条件。根据确定的发酵条件生产的除硫菌剂,经平板活菌计数法测定的菌数可达2.2×108 CFU/mL。
Abstract:
Desulfurization bacteria N411 is a kind of high-temperature denitrifying bacteria that can remove sulfur efficiently. In order to improve the culture efficiency and fermentation level of N411,it was carried out studies on the optimization of industrial fermentation medium and production conditions. The basic medium was optimized by adjusting the proportion of carbon and nitrogen in the medium,and the optimal medium was obtained from the four sets of formulas. The effects of temperature,pH value,and liquid volume on the growth of sulfur removal bacteria were investigated to determine industrial fermentation conditions.According to the sulfur removal bacteria produced under the determined fermentation conditions,the number of bacteria can reach 2 .2× 108 CFU / mL by the plate viable bacteria counting method.

参考文献/References:

[1]吴立新,吴祖芳.短小芽孢杆菌脂肪酸羟基化发酵特性与培养条件优化[J].食品与生物技术学报,2011,30(4):602-608.
[2]欧平,梁静娟,庞宗文.一株产大豆蛋白酶的芽孢杆菌发酵条件的优化[J].食品研究与开发,2011,32(2):133-139.
[3]范超,黎继烈,吴浩,等.重组巨大芽孢杆菌产青霉素G酰化酶发酵条件研究[J].中南林业科技大学学报,2011,31(7):124-129,135.
[4]纪明山,王毅婧.地衣芽孢杆菌生防菌株SDYT-79发酵条件优化[J].沈阳农业大学学报,2011,42(2):164-169.

备注/Memo

备注/Memo:
收稿日期:2021-04-20;改回日期:2021-08-13。
第一作者简介:杨帆(1983—),女,副研究员,从事油田微生物技术研究。E-mail:yangfan.jsyt@sinopec.com。
更新日期/Last Update: 1900-01-01